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Cells were diluted to 1 10 8 cells/ml in CTT, and 10 l were spotted onto 1/2 CTT plates with 1.5% agar, and either 200 M BCS or 300 M CuSO 4

However, in our analyses of 13 C-glucose, we found that total and labelled succinate accumulated to higher levels in mutant cells than in Gclc fl/fl FoB (Fig

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